Journal: Pharmaceutics

Article Title: Photodisruption of the Inner Limiting Membrane: Exploring ICG Loaded Nanoparticles as Photosensitizers

doi: 10.3390/pharmaceutics14081716

Figure Lengend Snippet: ( A ) Schematic overview of IVT injection of ICG NPs. ( B ) After migration through the vitreous, ICG NPs accumulate at the level of the ILM. Application of high intensity laser pulses results in VNB formation. Upon collapse of these VNBs, mechanical effects can disrupt the ILM paving the way for therapeutics to enter the retina. Image created with BioRender.

Article Snippet: In order to pursuit localized disruption effects at the level of the ILM, optimization of a suitable ICG-nanocarrier is the first pivotal step.

Techniques: Injection, Migration

Journal: Pharmaceutics

Article Title: Photodisruption of the Inner Limiting Membrane: Exploring ICG Loaded Nanoparticles as Photosensitizers

doi: 10.3390/pharmaceutics14081716

Figure Lengend Snippet: Dark field microscopy images of patient-derived isolated human ILM incubated with ICG NPs before and during application of a laser pulse (561 nm, ~ 3.6 J/cm 2 ). White arrowheads indicate presence of VNBs.

Article Snippet: In order to pursuit localized disruption effects at the level of the ILM, optimization of a suitable ICG-nanocarrier is the first pivotal step.

Techniques: Microscopy, Derivative Assay, Isolation, Incubation

Journal: Pharmaceutics

Article Title: Photodisruption of the Inner Limiting Membrane: Exploring ICG Loaded Nanoparticles as Photosensitizers

doi: 10.3390/pharmaceutics14081716

Figure Lengend Snippet: Visualization of retinal cryosections via confocal microscopy. ( A ) Control samples: untreated and laser treated. ( B ) Treated samples: application of ICG liposomes on top of bovine retinal explants followed by laser treatment with 800 nm pulsed laser light. Blue: Hoechst staining to visualize nuclei. Red: immunostaining for Collagen IV to show the ILM and blood vessels. White dotted line indicates complete absence of the ILM. Scale bar = 20 µm.

Article Snippet: In order to pursuit localized disruption effects at the level of the ILM, optimization of a suitable ICG-nanocarrier is the first pivotal step.

Techniques: Confocal Microscopy, Control, Liposomes, Staining, Immunostaining

Journal: Scientific Reports

Article Title: Ophthalmologists’ assessment of the handling characteristics of the novel Finesse Reflex Handle in comparison to those of a conventional handle

doi: 10.1038/s41598-024-56501-8

Figure Lengend Snippet: The two types of internal limiting membrane (ILM) forceps handles analysed in this study. We compared the Finesse Reflex Handle (upper device) with the Grieshaber Revolution Handle (lower device) as a conventional ILM forceps handle. The same 27-gauge MaxGrip forceps are attached to the tips of both handles as an integrated product. The widths of the grip portions (i.e., baskets) of Reflex and the conventional handle are 18 mm and 23 mm, respectively.

Article Snippet: A vitreous port was placed in the model eye, and ILM COAT (a water-soluble ILM-like substance; Bioniko) was coated onto the posterior pole of the model eye in accordance with the instructions provided by Bioniko (Fig. ).

Techniques: Membrane

Journal: Scientific Reports

Article Title: Ophthalmologists’ assessment of the handling characteristics of the novel Finesse Reflex Handle in comparison to those of a conventional handle

doi: 10.1038/s41598-024-56501-8

Figure Lengend Snippet: ILM peeling procedure. Left panel: blue-coloured ILM COAT (yellow arrowhead) is applied to the posterior pole of the fundus. Right panel: the participants peel the ILM COAT at the site of their preference.

Article Snippet: A vitreous port was placed in the model eye, and ILM COAT (a water-soluble ILM-like substance; Bioniko) was coated onto the posterior pole of the model eye in accordance with the instructions provided by Bioniko (Fig. ).

Techniques: